GENOME, SECRETOME AND GLUCOSE TRANSPORT HIGHLIGHT UNIQUE FEATURES OF THE PROTEIN PRODUCTION HOST PICHIA PASTORIS

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

Abstract Background Pichia pastoris is widely used as a production platform for heterologous proteins and model organism for organelle proliferation.Without a published genome sequence available, strain and process development relied mainly on analogies to other, well studied yeasts like Saccharomyces cerevisiae.Results To investigate specific feat

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Explicit kinetic heterogeneity: mathematical models for interpretation of deuterium labeling of heterogeneous cell populations.

Estimation of division and death rates of lymphocytes in different conditions is vital for quantitative understanding of the immune system.Deuterium, in the form of deuterated glucose or heavy water, can be used to measure rates of proliferation and death of lymphocytes in vivo.Inferring these rates from labeling and delabeling curves has been subj

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